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KMID : 1134820200490020127
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Journal of the Korean Society of Food Science and Nutrition
2020 Volume.49 No. 2 p.127 ~ p.133
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Cytoprotective Effects of Crude Polysaccharides Separated from Annona muricata Leaf in Macrophage
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Byun Eui-Baek
Kim Kwang-Wook
Kim Woo-Sik
Byun Eui-Hong
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Abstract
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This study focused on the macrophage protection effects of Annona muricata leaf polysaccharides (ALPS). Macrophage apoptosis was induced by a staurosporine (STS) treatment. STS induced macrophage apoptosis because of the cytotoxicity, caspase activation, reactive oxygen species (ROS) production, and increase in mitochondrial outer membrane permeability (MOMP). The inhibition of macrophage apoptosis by the ALPS treatment was confirmed. The ALPS treatment reduced STS-induced cell death and Annexin V positive cell levels significantly in a dose-dependent manner. In addition, the ALPS treatment inhibited the activation of caspase-3, 8, 9, and PARP in macrophages. Furthermore, the ALPS treatment reduced the STS-induced ROS production and MOMP increase in macrophages. Therefore, the ALPS treatment inhibits STS-induced macrophage apoptosis through the inhibition of cytotoxicity, caspase activation, ROS production, and MOMP increase, suggesting that ALPS could be a potential candidate as an immune cell protection drug.
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KEYWORD
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Annona muricata, macrophage, apoptosis, cytoprotective effects
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